Objectives: To explore the difference in odontoblast differentiation capacity between stem cells from human exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSCs), and to examine the expression level of ephrinB1 in odontoblast differentiation of these stem cells.
Methods: The stems cells were divided into a SHED group and a DPSCs group. After odontoblast differentiation induction, the above 2 groups were also randomly divided into a 3 d group and a 7 d group, respectively.The calcium deposition was detected by alkaline phosphatase (ALP) staining and alizarin red staining.The mRNA and protein expressions of ephrinB1, dentin matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) were detected by real-time PCR and Western blotting.
Results: ALP staining and alizarin red staining showed that there was stronger mineralization capacity in the SHED group than that in the DPSCs group. The relative mRNA and protein expressions of DMP-1, DSPP, and ephrinB1 in the SHED group were higher than those in the DPSCs group except for the protein expression of DMP-1 in the SHED 3 d group (all P<0.05).
Conclusions: SHED has stronger odontoblast differentiation capacity than DPSCs. In addition, ephrinB1 may be involved in the processes of odontoblast differentiation in the SHED and DPSCs.
目的: 探讨人脱落乳牙牙髓干细胞(stem cells from human exfoliated deciduous teeth，SHED)和恒牙牙髓干细胞(dental pulp stem cells，DPSCs)成牙本质分化能力的差异，以及肝配蛋白B1(ephrinB1) 的表达水平在两种细胞成牙本质分化过程中的变化。方法: 将牙髓干细胞分为SHED组和DPSCs组，对两组细胞进行成牙本质诱导，再将SHED组和DPSCs组分别随机分为3 d组和7 d组。采用碱性磷酸酯酶(alkaline phosphatase，ALP)染色和茜素红染色检测钙盐沉积；real-time PCR和蛋白质印迹法分别检测ephrinB1以及牙本质基质蛋白-1(dentin matrix protein-1，DMP-1)和牙本质涎磷蛋白(dentin sialophosphoprotein，DSPP)的mRNA和蛋白质表达。结果: SHED组ALP染色和茜素红染色均明显深于DPSCs组；除SHED 3 d组DMP-1的蛋白质表达外，SHED组DMP-1，DSPP和ephrinB1的mRNA和蛋白质表达水平均高于DPSCs组(均P<0.05)。结论: SHED较DPSCs具有更强的成牙本质分化能力，ephrinB1可能参与SHED和DPSCs成牙本质分化的调节。.
Keywords: dental pulp stem cells; ephrinB1; odontoblast differentiation; stem cells from human exfoliated deciduous teeth.