The 16S rRNA gene is the most commonly used molecular marker for identifying microorganisms. It is used in sequencing technology, including the first-generation, the second-generation, and the third-generation sequencing technology. A large number of studies on the 16S rRNA gene have contributed to a deeper understanding of oral microbial diversity. In the healthy oral cavity, there is microbial diversity in time and space. With the occurrence or development of oral diseases such as caries, periodontal disease, or halitosis, the microbial diversity will be changed.
16S核糖体RNA(16S ribosomal RNA，16S rRNA)基因是鉴定微生物最常用的分子标志物，被应用于第1代、第2代和第3代测序技术。大量关于16S rRNA基因的研究使人类对口腔微生物多样性的理解更加深入。在健康口腔中，微生物多样性在不同的时间或空间具有差异性。随着口腔疾病(如龋病、牙周病或口臭)的发生或发展，微生物多样性将发生改变。.
Keywords: 16S rRNA genes; microbial diversity; sequencing technology.