Immobilization of formate dehydrogenase on polyethylenimine-grafted graphene oxide with kinetics and stability study

Peng Lin; Yonghui Zhang; Guangxiao Yao; Heyu Huo; Hong Ren; Yixuan Wang; Shizhen Wang; Baishan Fang

doi:10.1002/elsc.201900134

Immobilization of formate dehydrogenase on polyethylenimine-grafted graphene oxide with kinetics and stability study

Eng Life Sci. 2019 Dec 1;20(3-4):104-111. doi: 10.1002/elsc.201900134. eCollection 2020 Mar.

Authors

Peng Lin¹, Yonghui Zhang^{1

2}, Guangxiao Yao¹, Heyu Huo¹, Hong Ren¹, Yixuan Wang¹, Shizhen Wang^{1

3}, Baishan Fang^{1

3}

Affiliations

¹ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering Xiamen University Xiamen P. R. China.
² College of Food and Biology Engineering Jimei University Xiamen P. R. China.
³ The Key Lab for Synthetic Biotechnology of Xiamen City Xiamen University Xiamen Fujian P. R. China.

Abstract

Graphene oxide-based nanomaterials are promising for enzyme immobilization due to the possibilities of functionalizing surface. Polyethylenimine-grafted graphene oxide was constructed as a novel scaffold for immobilization of formate dehydrogenase. Compared with free formate dehydrogenase and graphene oxide adsorbed formate dehydrogenase, thermostability, storage stability, and reusability of polyethylenimine-grafted graphene oxide-formate dehydrogenase were enhanced. Typically, polyethylenimine-grafted graphene oxide-formate dehydrogenase remained 47.4% activity after eight times' repeat reaction. The immobilized capacity of the polyethylenimine-grafted graphene oxide was 2.4-folds of that of graphene oxide. Morphological and functional analysis of polyethylenimine-grafted graphene oxide-formate dehydrogenase was performed and the assembling mechanism based on multi-level interactions was studied. Consequently, this practical and facile strategy will likely find applications in biosynthesis, biosensing, and biomedical engineering.

Keywords: assembly; formate dehydrogenase; graphene oxide; immobilization; polyethylenimine.