Objective: To understand the molecular characteristics and correlation among isolated strains of Brucella melitensis (BM) so as to improve the strategies on prevention and control of the disease in Jiangxi province. Methods: A total of 25 strains of BM isolated from human in 17 counties of Jiangxi province were analyzed by multiple locus variable-number tandem repeat analysis (MLVA) method. Results: A total of 25 strains of BM were classified into 24 independent genotypes with similarities between 67.00% and 100.00% and Simpson index between 0.000 and 0.773. There were 3 genotypes in MLVA8, including 60.00% (15/25) as 42 genotype, 32.00% (8/25) as 43 genotype, and 8.00% (2/25) as 63 genotype, respectively. There were 7 genotypes in MLVA11 identified, with 116 genotype and 125 genotype the main genotypes, accounting for 56.00% (14/25) of all the identified strains. Conclusions: Genes from all the 25 strains of BM that isolated from human being were with high genetic diversities, and various, genotypes. However, no obvious epidemiological correlation was noticed among these strains, indicating the complexity of the source of infection on Brucella in Jiangxi province.
目的： 掌握江西省羊种布鲁氏菌分子特征，了解分离菌株流行病学间相关性。 方法： 采用多位点可变数目串联重复序列分析方法（MLVA）对江西省17个县（区）25株人分离羊种布鲁氏菌进行分析。 结果： 25株羊种布鲁氏菌聚类可分为24个独立基因型，相似度为67.00%~100.00%，辛普森指数在0.000~0.773之间，MLVA8型有3个基因型，60.00%（15/25）为42型，32.00%（8/25）为43型，8.00%（2/25）为63型。MLVA11型有7个基因型，116、125型为主要基因型，两型菌株占56.00%（14/25）。 结论： 25株人分离羊种布鲁氏菌基因具有高度遗传多样性，菌株基因型繁多，各菌株间无明显流行病学相关性，表明江西省羊种布鲁氏菌传染来源复杂。.
Keywords: Brucella melitensis; Multiple locus variable-number tandem repeat analysis; Simpson index; Type.