The Use of Gapmers for In Vivo Suppression of Hepatic mRNA Targets

Methods Mol Biol. 2020:2176:177-184. doi: 10.1007/978-1-0716-0771-8_13.

Abstract

This chapter describes the use of locked nucleic acid (LNA) GapmeRs for the in vivo knockdown of specific mRNAs in the mouse liver and phenotype analysis. LNA GapmeRs may be tested for efficacy by transfection in cultured cells. They are delivered into mice in vivo by intravenous tail injection .

Keywords: Diet-induced obese mice; In vivo knockdown; LNA gapmers; Liver mRNA; Steatosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytochrome P-450 CYP2E1 / genetics
  • Forkhead Box Protein O3 / genetics
  • Gene Expression Regulation
  • Gene Knockdown Techniques / methods*
  • Injections, Intravenous
  • Liver / physiology*
  • Male
  • Mice, Inbred C57BL
  • MicroRNAs / genetics
  • Obesity / etiology
  • Obesity / genetics
  • Oligonucleotides / administration & dosage
  • Oligonucleotides / genetics*
  • PTEN Phosphohydrolase / genetics
  • Sirtuin 1 / genetics
  • Tail

Substances

  • Forkhead Box Protein O3
  • FoxO3 protein, mouse
  • MIRN132 microRNA, mouse
  • MicroRNAs
  • Oligonucleotides
  • locked nucleic acid
  • Cytochrome P-450 CYP2E1
  • cytochrome P-450 2E1, mouse
  • PTEN Phosphohydrolase
  • Pten protein, mouse
  • Sirt1 protein, mouse
  • Sirtuin 1