Dorsoventral inversion of the air-filled organ (lungs, gas bladder) in vertebrates: RNAsequencing of laser capture microdissected embryonic tissue

Emily Funk; Ezra Lencer; Amy McCune

doi:10.1002/jez.b.22998

Dorsoventral inversion of the air-filled organ (lungs, gas bladder) in vertebrates: RNAsequencing of laser capture microdissected embryonic tissue

J Exp Zool B Mol Dev Evol. 2020 Sep;334(6):325-338. doi: 10.1002/jez.b.22998. Epub 2020 Aug 30.

Authors

Emily Funk^{1

2}, Ezra Lencer³, Amy McCune¹

Affiliations

¹ Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York, USA.
² Animal Science Department, University of California Davis, Genomic Variation Lab, Davis, California, USA.
³ Department of Craniofacial Biology, University of Colorado Denver-Anschutz Medical Campus, Aurora, Colorado, USA.

Abstract

How modification of gene expression generates novel traits is key to understanding the evolutionary process. We investigated the genetic basis for the origin of the piscine gas bladder from lungs of ancestral bony vertebrates. Distinguishing these homologous organs is the direction of budding from the foregut during development; lungs bud ventrally and the gas bladder buds dorsally.

Keywords: Tbx5; dorsoventral; gas bladder; lungs.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Air Sacs / embryology*
Animals
Biological Evolution*
Fishes / embryology*
Gene Expression Regulation, Developmental / physiology
Laser Capture Microdissection*
Lung / embryology*
Sequence Analysis, RNA
Vertebrates

Grants and funding

T32 CA174648/CA/NCI NIH HHS/United States