A molecularly imprinted polymer-modified potentiometric histamine (HIS) sensor was prepared and used for quantitative determination of HIS in bovine serum. The calibration curve using the potential responses measured in 1 × 10-3 mol L-1 phosphate buffer (pH 7.4) showed good linearity in the HIS concentration range of 3 × 10-4 to 1 × 10-2 mol L-1 (r = 0.92), with a detection limit of 1.6 × 10-4 mol L-1. In bovine serum samples, the HIS sensor showed good recovery values of 91 - 104%. Therefore, this HIS sensor successfully determined the HIS concentration in bovine serum samples.
Keywords: Potentiometric sensor; histamine; molecularly imprinted polymer; quantitative determination; selectivity; serum.