This study was designed to analyze the pharmacological effects of Typha domingenesis crude 70% aqueous-ethanol extract of Typha domingensis (Td. Cr) in gastrointestinal, respiratory and vascular diseases. Rabbits (2.0-3.0 kg) and BALB/c mice (20-40 g) of local breed have been used as experimental animals using the established methodologies from literature with slight modification. The findings suggested that Typha domingensis caused complete relaxation of spontaneous and K+ (80mM)-induced contractions in isolated rabbit jejunum. Rightward parallel shift of calcium concentration response curves was observed. Typha domingensis exhibited relaxant effect on Carbachol (Cch)-induced contractions in isolated rabbit tracheal preparations. Furthermore, Typha domingensis caused relaxation of phenylephrine (1μM)- induced contractions in isolated rabbit aorta preparations. These effects were similar to verapamil, a standard calcium channel blocker. These findings could be the basis for explaining the spasmolytic, bronchodilator and vasodilator activities of the extract, through a possible calcium channel blocking activity.