Inner filter effect as a sensitive sensing platform for detection of nitrofurantoin using luminescent drug-based carbon nanodots

Abstract

In the present paper, a sensitive and selective inner filter effect sensing platform was designed to detect nitrofurantoin (NIT) in pharmaceutical dosage form. Nitrogen and phosphorus co-doped carbon nanodots (CNDs) prepared via solvothermal treatment of folic acid and phosphoric acid. The prepared CNDs exhibit greenish fluorescence at 470 nm when excited at 340 nm with fluorescence quantum yield up to 40%. The CNDs exhibit high stability in various pH, temperature, and ionic strength which adds valuable merits to its pharmaceutical applications. The emission is quenched in the presence of absorber (here NIT) while the fluorophores were not quench by the presence of common pharmaceutical excipients. A fluorometric assay was fabricated to determine NIT in capsules by quenching of the CNDs. The linear response for the proposed method was from 5.0 μM to 90 μM with the detection limit being 1.4 μM. To validate the method, the recovery of NIT in spiked sample was measured which was 96.6% -103.3%. The method was applied to the determination of NIT in pharmaceutical capsule samples, with comparable results of a reference method stated by the British Pharmacopeia (BP). Furthermore, the sub-acute toxicity studies of CNDs were investigated using normal male Balb/c mice forcefully drunk with 3 different dosages of CNDs. Animals did not produce treatment related signs of toxicity or mortality in any of the animals tested during the 28 days observation period. Additionally, no significant (P > 0.05) changes in the body weight, haematological and biochemical parameters compared with the control group were not revealed. Similarly, histopathological examination of the internal vital organs did not show any morphological alterations.

Keywords: Carbon nanodots; Cytotoxicity of carbon dots, pharmaceutical probe; Fluorescence sensor; Inner filter effect; Nitrofurantoin.