EPR Spectroscopy as a Method for ROS Quantification in the Skin

Methods Mol Biol. 2021:2202:137-148. doi: 10.1007/978-1-0716-0896-8_12.

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for human life as it stimulates vitamin D synthesis and well-being. However, an excessive dose of solar radiation leads to the formation of free radicals. Here, we describe an EPR method for measuring the amount of radicals induced by UVA irradiation in excised skin. For the first time, a wavelength stable UVA LED (365 nm) was used. The method allows the quantitative determination of radicals in skin before, during, and after UVA irradiation. A dose-dependent radical production could be demonstrated, independent of the yielded power.

Keywords: Cumulative radical production; Electron paramagnetic resonance (EPR) spectroscopy; Free radicals; Skin; Spin probe; UVA light.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cyclic N-Oxides / chemistry
  • Electron Spin Resonance Spectroscopy / methods*
  • Free Radicals / chemistry
  • Humans
  • Reactive Oxygen Species / analysis*
  • Skin / metabolism*
  • Spin Labels
  • Ultraviolet Rays

Substances

  • Cyclic N-Oxides
  • Free Radicals
  • Reactive Oxygen Species
  • Spin Labels