Dual-function membranes based on alginate/methyl cellulose composite for control drug release and proliferation enhancement of fibroblast cells

Hussien Ahmed Abbas; Mostafa Mabrouk; Ahmed A F Soliman; Hanan H Beherei

doi:10.1016/j.ijbiomac.2020.08.171

Dual-function membranes based on alginate/methyl cellulose composite for control drug release and proliferation enhancement of fibroblast cells

Int J Biol Macromol. 2020 Dec 1:164:2831-2841. doi: 10.1016/j.ijbiomac.2020.08.171. Epub 2020 Aug 25.

Authors

Hussien Ahmed Abbas¹, Mostafa Mabrouk², Ahmed A F Soliman³, Hanan H Beherei⁴

Affiliations

¹ Inorganic Chemistry Department, National Research Centre, El-Behouth Street, P.O. Box 12622, Dokki, Cairo, Egypt.
² Refractories, Ceramics and Building Materials Department, National Research Centre, 33El Bohouth St. (former EL Tahrir St.), Dokki, P.O.12622, Giza, Egypt. Electronic address: mostafamabrouk.nrc@gmail.com.
³ Pharmacognosy Department, Pharmaceutical and Drug Industries Division, National Research Centre (NRC), 33 El Behooth St. Dokki, P.O.12622, Giza, Egypt.
⁴ Refractories, Ceramics and Building Materials Department, National Research Centre, 33El Bohouth St. (former EL Tahrir St.), Dokki, P.O.12622, Giza, Egypt.

PMID: 32853615
DOI: 10.1016/j.ijbiomac.2020.08.171

Abstract

Membranes based on natural polymers are highly promising therapies for skin damaged sites as they can mimic its biological microstructure to support the fibroblasts cells survival and proliferation. In addition, these membranes could be loaded with active molecules that help in skin regeneration and eliminate the potential bacterial infection. This research aims to formulate novel medicated membranes for controlled release and cytocompatibility elevation of fibroblast cells for engineering of soft tissue. Pre-formulation researches have been conducted for membranes of sodium alginate (Alg)/methyl cellulose (MC) that used loaded with undoped, Bi doped and Bi, Cu co-doped SrTiO₃ using solvent casting technique. In addition, another group of these membranes were loaded with DOXycycline antibiotic (DOX) as model drug as well as for eliminating the potential bacterial infections. The prepared membranes were evaluated by XRD, SEM-EDX, FTIR, Zetasizer, and swelling behaviour was also tested. Profiles of the released drug were determined using phosphate-buffered saline (PBS) (pH 7.4) at 37 °C for 30 days. The investigation of the cytocompatibility and proliferation of fibroblast cells with the prepared membranes were conducted. The XRD, FTIR and SEM data recognised the possible interaction that takes place among Alg and MC, through presence of hydrogen bonds. Existence of the nano-particles within the membrane polymer matrix enhanced the membrane stability and enhanced the drug release rate (from 20 to 45%). Medication release mechanism elucidated that DOX was released from all the fabricated membranes through the relaxation of polymer matrix that takes place after swelling. The filler type and/or dopant type possess no remarkable influence on the cytotoxicity of the membranes against the investigated cells when compared to their individual influence on the same cells. Cells attachments results have revealed an impressive effect for DOX-loaded membranes on the cells affinity and growth. These membranes are recommended for treatments of skin infections.

Keywords: Controlled drug release; Cytocompatibility; DOXycycline; Dual-function membranes; Natural polymers.

MeSH terms

Alginates / chemistry*
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Cell Line
Cell Proliferation / drug effects
Cell Survival / drug effects
Doxycycline / chemistry
Doxycycline / pharmacology*
Drug Liberation
Fibroblasts / cytology*
Fibroblasts / drug effects
Humans
Hydrogen Bonding
Methylcellulose / chemistry*
Microbial Sensitivity Tests
Nanoparticles
Particle Size

Substances

Alginates
Anti-Bacterial Agents
Methylcellulose
Doxycycline