Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems

Ksenia M Shakirova; Viktoriia Y Ovchinnikova; Erdem B Dashinimaev

doi:10.3389/fbioe.2020.00882

Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems

Front Bioeng Biotechnol. 2020 Jul 28:8:882. doi: 10.3389/fbioe.2020.00882. eCollection 2020.

Authors

Ksenia M Shakirova^{1

2

3}, Viktoriia Y Ovchinnikova^{1

3}, Erdem B Dashinimaev^{2

3}

Affiliations

¹ Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
² Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
³ Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, Russia.

Abstract

The speed of reprogramming technologies evolution is rising dramatically in modern science. Both the scientific community and health workers depend on such developments due to the lack of safe autogenic cells and tissues for regenerative medicine, genome editing tools and reliable screening techniques. To perform experiments efficiently and to propel the fundamental science it is important to keep up with novel modifications and techniques that are being discovered almost weekly. One of them is CRISPR/Cas9 based genome and transcriptome editing. The aim of this article is to summarize currently existing CRISPR/Cas9 applications for cell reprogramming, mainly, to compare them with other non-CRISPR approaches and to highlight future perspectives and opportunities.

Keywords: CRISPR/Cas9; dCas9; genome screening; human cell reprogramming; regenerative medicine; transactivator systems.

Publication types

Review