Hydrogen Peroxide Production by the Spot-Like Mode Action of Bisphenol A

Ioannis-Dimosthenis S Adamakis; Ilektra Sperdouli; Eleftherios P Eleftheriou; Michael Moustakas

doi:10.3389/fpls.2020.01196

Hydrogen Peroxide Production by the Spot-Like Mode Action of Bisphenol A

Front Plant Sci. 2020 Aug 5:11:1196. doi: 10.3389/fpls.2020.01196. eCollection 2020.

Authors

Ioannis-Dimosthenis S Adamakis¹, Ilektra Sperdouli², Eleftherios P Eleftheriou³, Michael Moustakas³

Affiliations

¹ Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece.
² Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, Thessaloniki, Greece.
³ Department of Botany, Aristotle University of Thessaloniki, Thessaloniki, Greece.

Abstract

Bisphenol A (BPA), an intermediate chemical used for synthesizing polycarbonate plastics, has now become a wide spread organic pollutant. It percolates from a variety of sources, and plants are among the first organisms to encounter, absorb, and metabolize it, while its toxic effects are not yet fully known. Therefore, we experimentally studied the effects of aqueous BPA solutions (50 and 100 mg L^-1, for 6, 12, and 24 h) on photosystem II (PSII) functionality and evaluated the role of reactive oxygen species (ROS) on detached leaves of the model plant Arabidopsis thaliana. Chlorophyll fluorescence imaging analysis revealed a spatiotemporal heterogeneity in the quantum yields of light energy partitioning at PSII in Arabidopsis leaves exposed to BPA. Under low light PSII function was negatively influenced only at the spot-affected BPA zone in a dose- and time-dependent manner, while at the whole leaf only the maximum photochemical efficiency (Fv/Fm) was negatively affected. However, under high light all PSII photosynthetic parameters measured were negatively affected by BPA application, in a time-dependent manner. The affected leaf areas by the spot-like mode of BPA action showed reduced chlorophyll autofluorescence and increased accumulation of hydrogen peroxide (H₂O₂). When H₂O₂ was scavenged via N-acetylcysteine under BPA exposure, PSII functionality was suspended, while H₂O₂ scavenging under non-stress had more detrimental effects on PSII function than BPA alone. It can be concluded that the necrotic death-like spots under BPA exposure could be due to ROS accumulation, but also H₂O₂ generation seems to play a role in the leaf response against BPA-related stress conditions.

Keywords: H2O2 scavenger; bisphenol A; chlorophyll fluorescence imaging; necrotic death-like spot; photosystem II functionality; plastoquinone pool; reactive oxygen species; signaling molecule.