Antiproliferative activity of green, black tea and olive leaves polyphenols subjected to biosorption and in vitro gastrointestinal digestion in Caco-2 cells

Hanène Jilani; Antonio Cilla; Reyes Barberá; Moktar Hamdi

doi:10.1016/j.foodres.2020.109317

Antiproliferative activity of green, black tea and olive leaves polyphenols subjected to biosorption and in vitro gastrointestinal digestion in Caco-2 cells

Food Res Int. 2020 Oct:136:109317. doi: 10.1016/j.foodres.2020.109317. Epub 2020 May 16.

Authors

Hanène Jilani¹, Antonio Cilla², Reyes Barberá³, Moktar Hamdi⁴

Affiliations

¹ Laboratory of Microbial Ecology and Technology, Department of Biological and Chemical Engineering, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Centre Urbain Nord, 2 Boulevard de la Terre, B.P. 676, 1080 Tunis, Tunisia; Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. Electronic address: jilanihanene@yahoo.fr.
² Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. Electronic address: antonio.cilla@uv.es.
³ Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
⁴ Laboratory of Microbial Ecology and Technology, Department of Biological and Chemical Engineering, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Centre Urbain Nord, 2 Boulevard de la Terre, B.P. 676, 1080 Tunis, Tunisia.

PMID: 32846525
DOI: 10.1016/j.foodres.2020.109317

Abstract

Olive (Olea europaea L.) leaves and tea (Camellia sinensis) are rich sources of bioactive compounds, especially polyphenols. Our previous studies have evidenced the potential use of Saccharomyces cerevisiae as a natural delivery system for these antioxidants and a means to improve their bioaccessibility in the human gut. In the present work, the antiproliferative effect of green tea (GT), black tea (BT) and olive leaves (OL) infusions and suspensions of S. cerevisiae were evaluated, for the first time, in human colon cancer cells (Caco-2) after biosorption and in vitro gastrointestinal digestion. The bioaccessible fractions (BF) were not overtly cytotoxic, not affecting cell viability. ROS and mitochondrial membrane potential changes (Δψm) values were reduced compared with control cells. Moreover, all the BF after biosorption induced a significant (p < 0.05) increase in cell proportions in S-phase. The arrest of the cell cycle was reversible without induction of apoptosis, suggesting that the biosorbed phenolics in both infusions and suspensions act as cytostatic agents.

Keywords: Bioaccessible fractions; Biosorption; Caco-2 cells; Olive leaves; Polyphenols; Saccharomyces cerevisiae; Tea.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects
Biological Availability
Caco-2 Cells
Camellia sinensis / chemistry*
Cell Cycle / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects
Digestion*
Humans
Olea / chemistry*
Plant Leaves / chemistry*
Polyphenols / metabolism
Polyphenols / pharmacokinetics
Polyphenols / pharmacology*
Saccharomyces cerevisiae / metabolism
Tea / chemistry

Substances

Polyphenols
Tea