First Observation of an Acetate Switch in a Methanogenic Autotroph (Methanococcus maripaludis S2)

Chi Hung Vo; Nishu Goyal; Iftekhar A Karimi; Markus Kraft

doi:10.1177/1178636120945300

First Observation of an Acetate Switch in a Methanogenic Autotroph (Methanococcus maripaludis S2)

Microbiol Insights. 2020 Jul 31:13:1178636120945300. doi: 10.1177/1178636120945300. eCollection 2020.

Authors

Chi Hung Vo^{1

2}, Nishu Goyal³, Iftekhar A Karimi^{1

2}, Markus Kraft^{2

4

5}

Affiliations

¹ Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore.
² Cambridge Centre for Advanced Research and Education in Singapore Ltd, Singapore.
³ Department of Chemical Engineering, University of Petroleum and Energy Studies, Dehradun, India.
⁴ Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
⁵ School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.

Abstract

The transition from acetate production by a microorganism in its early growth phase to acetate re-uptake in its late growth phase has been termed acetate switch. It has been observed in several heterotrophic prokaryotes, but not in an autotroph. Furthermore, all reports hitherto have involved the tricarboxylic acid cycle. This study reports the first observation of acetate switch in a methanogenic autotroph Methanococcus maripaludis S2, which uses the Wolfe cycle for its anaerobic respiration. When grown in minimal medium with carbon dioxide as the sole carbon source, and either ammonium or dinitrogen as the sole nitrogen source, M. maripaludis S2 dissimilated acetate in the early growth phase and assimilated it back in the late growth phase. The acetate switch was more pronounced in the dinitrogen-grown cultures. We postulate that the acetate dissimilation in M. maripaludis S2 may serve as a metabolic outlet for the carbon overflow in the early growth phase, and the assimilation in the late growth phase may be due to the scarcity of the carbon source. Based on the primary and secondary protein structures, we propose that MMP0253 may function as the adenosine diphosphate (ADP)-forming acetyl-CoA synthetase to catalyse acetate formation from acetyl-CoA. To verify this, we produced MMP0253 via the ligation-independent cloning technique in Escherichia coli strain Rosetta (DE3) using pNIC28-Bsa4 as the vector. The recombinant protein showed catalytic activity, when added into a mixture of acetyl-CoA, ADP, and inorganic phosphate (P_i). The concentration profile of acetate, together with the enzymatic activity of MMP0253, shows that M. maripaludis S2 can produce acetate and exhibit an acetate switch.

Keywords: ADP-forming acetyl-CoA synthetase; Acd; Methanococcus maripaludis; acetate assimilation; acetate dissimilation; acetate switch.