Infections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) were attached to the surface of a titanium alloy, Ti6Al4V, by conjugation to a polydopamine linking substrate. The topography of the polydopamine coating was evaluated by electron microscopy and coating thickness measurements were performed with ellipsometry and Atomic Force Microscopy (AFM). The subsequently attached peptide stability was investigated with release profile studies in simulated body fluid, using both fluorescence imaging and High-Performance Liquid Chromatography (HPLC). Finally, the hydrophobicity of the coating was characterised by water contact angle measurements. The designed AMPs were shown to provide long-term bonding to the polydopamine-coated Ti6Al4V surfaces.
Keywords: KR-12; Ti6Al4V; antimicrobial peptides; cathelicidin; polydopamine.