Branch Migration Activity of Rad54 Protein

Methods Mol Biol. 2021:2153:145-167. doi: 10.1007/978-1-0716-0644-5_11.

Abstract

Rad54 is a eukaryotic protein that plays an important role in homologous recombination. Rad54, a member of the Swi2/Snf2 family, binds to Holliday junctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not.

Keywords: Branch migration; Holliday junctions; Homologous recombination; Rad54 protein.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • DNA / chemistry
  • DNA / metabolism*
  • DNA Breaks, Double-Stranded
  • DNA Helicases / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Hydrolysis
  • Meiosis

Substances

  • DNA-Binding Proteins
  • Adenosine Triphosphate
  • DNA
  • DNA Helicases
  • RAD54L protein, human