Tephrosia purpurea stem extract fractions shows more amount of Cyclohexane, 1-ethenyl-1-methyl-2,4-bis(1-methylethenyl)-, (1α,2β,4β)- (62.32%). Secondary metabolites contents were higher in stem than root of T. purpurea. It is the first time a chalcone (Pongamol) was crystallised from the column chromatography fraction of T. purpurea stem extract identified by using FT-IR and XRD. Quantitative analysis of T. purpurea stem and root reveals that total saponins, tannins and phenols (mg/g plant powder) contents were higher in the former than the latter part of the plant, respectively.
Keywords: FT-IR; GC-MS; Phytochemicals; Pongamol; XRD; crystal.