In vivo monitoring of carbonic anhydrase expression during the growth of larval zebrafish: a new environment-sensitive fluorophore for responsive turn-on fluorescence

Chih-Hung Chou; Chang-Yi Wu; Chun-Lin Chen; Jun-Qing Zhou; Yu-Chen Kao; Hsing-Yin Chen; Po-Chiao Lin

doi:10.1039/d0cc03090b

In vivo monitoring of carbonic anhydrase expression during the growth of larval zebrafish: a new environment-sensitive fluorophore for responsive turn-on fluorescence

Chem Commun (Camb). 2020 Sep 28;56(76):11307-11310. doi: 10.1039/d0cc03090b. Epub 2020 Aug 25.

Authors

Chih-Hung Chou¹, Chang-Yi Wu, Chun-Lin Chen, Jun-Qing Zhou, Yu-Chen Kao, Hsing-Yin Chen, Po-Chiao Lin

Affiliation

¹ Department of Chemistry, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. pclin@mail.nsysu.edu.tw.

PMID: 32840276
DOI: 10.1039/d0cc03090b

Abstract

This study monitors the dynamic progress of a newly developed background-free, target responsive strategy; 2,3-dihydroquinolin-4-imine (DQI) that can instantly respond to environmental changes with fluorescence enhancement, revealing a comprehensive platform for in vivo fluorescence bioimaging of mebrane-bound carbonic anhydrase II in HeLa cells and its expression during the growth of larval zebrafish.

MeSH terms

Animals
Carbonic Anhydrase II / antagonists & inhibitors
Carbonic Anhydrase II / biosynthesis*
Carbonic Anhydrase II / metabolism
Carbonic Anhydrase Inhibitors / pharmacology
Density Functional Theory
Fluorescent Dyes / analysis*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Larva / enzymology
Larva / growth & development
Molecular Structure
Optical Imaging
Quinolines / chemical synthesis
Quinolines / chemistry*
Sulfonamides / pharmacology
Zebrafish / growth & development*

Substances

Carbonic Anhydrase Inhibitors
Fluorescent Dyes
Quinolines
Sulfonamides
Carbonic Anhydrase II
CA2 protein, human