Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control

Chembiochem. 2021 Jan 5;22(1):63-72. doi: 10.1002/cbic.202000423. Epub 2020 Nov 17.

Abstract

Conditional control of CRISPR/Cas9 has been developed by using a variety of different approaches, many focusing on manipulation of the Cas9 protein itself. However, more recent strategies for governing CRISPR/Cas9 function are based on guide RNA (gRNA) modifications. They include control of gRNAs by light, small molecules, proteins, and oligonucleotides. These designs have unique advantages compared to other approaches and have allowed precise regulation of gene editing and transcription. Here, we discuss strategies for conditional control of gRNA function and compare effectiveness of these methods.

Keywords: CRISPR; Cas9; gene editing; nucleic acid; optical control; protection groups.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • CRISPR-Associated Protein 9 / genetics
  • CRISPR-Associated Protein 9 / metabolism*
  • CRISPR-Cas Systems / genetics*
  • RNA, Guide, CRISPR-Cas Systems / genetics
  • RNA, Guide, CRISPR-Cas Systems / metabolism*

Substances

  • RNA, Guide, CRISPR-Cas Systems
  • CRISPR-Associated Protein 9