Visualizing Coronavirus Entry into Cells

Aleksandra Milewska; Katarzyna Owczarek; Artur Szczepanski; Krzysztof Pyrc

doi:10.1007/978-1-0716-0900-2_18

Visualizing Coronavirus Entry into Cells

Methods Mol Biol. 2020:2203:241-261. doi: 10.1007/978-1-0716-0900-2_18.

Authors

Aleksandra Milewska¹, Katarzyna Owczarek¹, Artur Szczepanski¹, Krzysztof Pyrc²

Affiliations

¹ Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
² Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. k.a.pyrc@uj.edu.pl.

PMID: 32833217
DOI: 10.1007/978-1-0716-0900-2_18

Abstract

Coronavirus entry encompasses the initial steps of infection, from virion attachment to genome release. Advances in fluorescent labeling of viral and cellular components and confocal imaging enable broad spectrum studies on this process. Here, we describe methods for visualization of coronavirus entry into immortalized cell lines and 3D tissue culture models.

Keywords: Confocal microscopy; Coronavirus; Endocytosis; Entry; Internalization.

MeSH terms

Cell Line
Coronavirus / isolation & purification
Coronavirus / pathogenicity*
Coronavirus Nucleocapsid Proteins
Culture Media / chemistry
Endocytosis
Host-Pathogen Interactions / physiology*
Humans
Microscopy, Confocal / methods*
Nucleocapsid Proteins / metabolism
Triiodobenzoic Acids / chemistry
Virus Internalization

Substances

Coronavirus Nucleocapsid Proteins
Culture Media
Nucleocapsid Proteins
Triiodobenzoic Acids
iodixanol