Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells

Hirohito Ishigaki; Yasushi Itoh

doi:10.1007/7651_2020_303

Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells

Methods Mol Biol. 2022:2454:625-641. doi: 10.1007/7651_2020_303.

Authors

Hirohito Ishigaki¹, Yasushi Itoh²

Affiliations

¹ Department of Pathology, Shiga University of Medical Science, Otsu, Shiga, Japan. ihirona2@gmail.com.
² Department of Pathology, Shiga University of Medical Science, Otsu, Shiga, Japan.

PMID: 32833131
DOI: 10.1007/7651_2020_303

Abstract

Induced pluripotent stem cells (iPSCs) are useful for the development of therapies in regenerative medicine, analysis of pathogenesis, and exploration of candidate drugs. We developed an alternative usage of iPSCs of which the MHC haplotype is matched to transplantable recipients in a cynomolgus macaque model. We established two cancer cell lines, embryonal carcinoma, and glioblastoma cell lines from cynomolgus monkey iPSCs. Here, we describe a method to induce the cancer cell lines including a technique for culture of the monkey iPSCs on feeder cells and the induction of hematopoietic stem cells and neural progenitor cells from monkey iPSCs.

Keywords: Cancer cell line; Cynomolgus macaque; Embryonal carcinoma; Glioblastoma; Oncogene; Retrovirus; iPSC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinoma, Embryonal* / metabolism
Cell Differentiation
Cell Line
Cells, Cultured
Glioblastoma* / metabolism
Induced Pluripotent Stem Cells*
Macaca fascicularis