Sulfated polymannuroguluronate TGC161 ameliorates leukopenia by inhibiting CD4+ T cell apoptosis

Chuanqin Shi; Wenwei Han; Meifang Zhang; Ruochen Zang; Kaixin Du; Li Li; Ximing Xu; Chunxia Li; Shixin Wang; Peiju Qiu; Huashi Guan; Jinbo Yang; Shuai Xiao; Xin Wang

doi:10.1016/j.carbpol.2020.116728

Sulfated polymannuroguluronate TGC161 ameliorates leukopenia by inhibiting CD4⁺ T cell apoptosis

Carbohydr Polym. 2020 Nov 1:247:116728. doi: 10.1016/j.carbpol.2020.116728. Epub 2020 Jul 6.

Authors

Chuanqin Shi¹, Wenwei Han¹, Meifang Zhang¹, Ruochen Zang¹, Kaixin Du², Li Li³, Ximing Xu³, Chunxia Li⁴, Shixin Wang⁴, Peiju Qiu³, Huashi Guan³, Jinbo Yang³, Shuai Xiao⁵, Xin Wang⁶

Affiliations

¹ Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China; Center for Innovation Marine Drug Screening & Evaluation, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China.
² Center for Innovation Marine Drug Screening & Evaluation, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China; Marine Biomedical Research Institute of Qingdao, Qingdao, 266071, China.
³ Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China; Center for Innovation Marine Drug Screening & Evaluation, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China; Marine Biomedical Research Institute of Qingdao, Qingdao, 266071, China.
⁴ Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China; Marine Biomedical Research Institute of Qingdao, Qingdao, 266071, China.
⁵ Department of Gastrointestinal Surgery and Institute of Clinical Medicine, the First Affiliated Hospital, University of South China, Hengyang, 421001, China. Electronic address: xiaoshuai1982@hotmail.com.
⁶ Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China; Center for Innovation Marine Drug Screening & Evaluation, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China; Marine Biomedical Research Institute of Qingdao, Qingdao, 266071, China. Electronic address: wx8399@ouc.edu.cn.

Abstract

Polysaccharides have aroused considerable interest due to their diverse biological activities and low toxicity. In this study, we evaluated the effect of marine polysaccharide sulfated polymannuroguluronate (TGC161) on the leukopenia induced by chemotherapy. It is found that TGC161 ameliorates the leukopenia. Besides, TGC161 would promote CD4⁺ T cell differentiation and maturation in the thymus, but does not have a significant effect on precursor cells in bone marrow. Furthermore, TGC161 inhibits CD4⁺ T cell apoptosis in vitro. Collectively, our findings offer a natural and harmless polysaccharide to ameliorate leukopenia.

Keywords: CD4(+)T cell apoptosis; Chemotherapy; Leukopenia; Sulfated polymannuroguluronate; TGC161.

MeSH terms

Animals
Apoptosis / drug effects*
CD4-Positive T-Lymphocytes / drug effects
CD4-Positive T-Lymphocytes / immunology*
Leukopenia / immunology
Leukopenia / pathology
Leukopenia / prevention & control*
Male
Mice
Mice, Inbred C57BL
Polysaccharides / pharmacology*

Substances

Polysaccharides
sulfated polymannuroguluronate