Anammox reactor exposure to thiocyanate: Long-term performance and microbial community dynamics

Abstract

Anaerobic ammonium oxidation (anammox) is an autotrophic denitrification process that has broad application potential for treating coking wastewaters. The present study estimated the effects of thiocyanate (SCN^-), a common pollutant in coking wastewaters, on anammox processes and microbial communities in anammox reactors for over two years of continuous exposure. The addition of SCN^- (from 50 to 200 mg L^-1) showed negative effects on the denitrification performance of the anammox reactors. In SCN^--dosed reactors, increased effluent ammonium concentrations indicated the occurrence of SCN^--based biodegradation processes. Microbial analysis revealed that the anammox species almost disappeared in the reactor dosed with SCN^- at over 100 mg L^-1. Instead, an abundance of chemolithoautotrophic bacteria belonging to the Thiobacillus genus demonstrated a linear increase with SCN^- addition. The competition between anammox species and SCN^--degrading microorganisms was expected to dominate the inhibition effects of SCN^- addition on the performance of anammox reactors.

Keywords: Anammox; Microbial community; Thiobacillus; Thiocyanate; Thiocyanate-based denitrification.