A highly selective fluorescent probe for the intracellular measurement of magnesium ion

Abstract

Magnesium ion (Mg⁺²) plays an important role in various biological processes. All the commercial indicators available share a common drawback, i.e., they have a higher affinity towards calcium ions (Ca⁺²) than Mg⁺². In this study, we reported a new robust green fluorescent indicator, Mag-520, for detection of Mg⁺² in live cells. Our results showed that Mag-520 has 10 fold higher affinity towards Mg⁺² than Ca⁺², while mag-fluo-4 has less than 0.5 fold affinity to Mg⁺² than Ca⁺² under the same conditions using flow cytometry and fluorescence microscopy. The results demonstrated that Mag-520 provides a better tool to measure Mg⁺² with less interference from Ca⁺².

Keywords: Flow cytometer; Fluorescence; Magnesium ion; Specificity; in vitro analysis.