Diel and seasonal vertical migrations of zooplankton represent a widespread phenomenon occurring in marine and freshwater environments. Diel migrations are panoceanic, while seasonal migrations usually occur in temperate and polar areas. This paper describes differences in the diel and seasonal vertical migrations in the Drake Passage north and south of the Polar Front (PF). We analyzed material of 85 stations collected in spring of 2008 and 2010 (October-November) and in summer of 2010 and 2011 (January) within the 0-300 m depth range during various time of a day. At each station we sampled the upper mixed (UL), the middle (ML), and the deeper layers (DL) bounded by hydrological gradients. Diel migrations were significantly different south and north of the PF in terms of total abundance, biomass, diversity and individual taxa density. In both seasons, mesoplankton dielly migrated between the ML/DL and the UL north of the PF and between layers below 300 m and the DL and ML south of the PF. Deeper range of diel migrations south of the PF was coupled with a general mesoplankton descent in summer period compared to spring. Conversely, north of the PF, mesoplankton ascended to upper layers in summer, which was mirrored in lesser depths of diel migrations. The differences in the plankton distribution on both sides of the PF are likely associated with variations of vertical distribution of phytoplankton. Some abundant taxa such as Aetideus sp. and Oithona plumifera showed both common (nighttime ascend) and inverted (nighttime descend) vertical migrations depending on season and position related to the PF.