Altering model cell membranes by means of localized magnetic heating

Silvia Ruiz-Rincón; Alejandro González-Orive; Valeria Grazú; Raluca M Fratila; Jesús M de la Fuente; Pilar Cea

doi:10.1016/j.colsurfb.2020.111315

Altering model cell membranes by means of localized magnetic heating

Colloids Surf B Biointerfaces. 2020 Dec:196:111315. doi: 10.1016/j.colsurfb.2020.111315. Epub 2020 Aug 9.

Authors

Silvia Ruiz-Rincón¹, Alejandro González-Orive², Valeria Grazú³, Raluca M Fratila³, Jesús M de la Fuente³, Pilar Cea⁴

Affiliations

¹ Instituto de Nanociencia de Aragón (INA), Campus Rio Ebro, Universidad de Zaragoza, C/Mariano Esquillor, s/n, 50018 Zaragoza, Spain; Laboratorio de Microscopias Avanzadas (LMA),Campus Río Ebro, Universidad de Zaragoza, C/Mariano Esquillor, s/n, 50018 Zaragoza, Spain; Instituto de Ciencia de Materiales de Aragón (ICMA), Universidad de Zaragoza-CSIC, C/Pedro Cerbuna 12, 50009 Zaragoza, Spain.
² Technical and Macromolecular Chemistry, University of Paderborn, Warburger Strasse 100, 33098 Paderborn, Germany.
³ Instituto de Ciencia de Materiales de Aragón (ICMA), Universidad de Zaragoza-CSIC, C/Pedro Cerbuna 12, 50009 Zaragoza, Spain; Networking Biomedical Research Centre of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
⁴ Instituto de Nanociencia de Aragón (INA), Campus Rio Ebro, Universidad de Zaragoza, C/Mariano Esquillor, s/n, 50018 Zaragoza, Spain; Laboratorio de Microscopias Avanzadas (LMA),Campus Río Ebro, Universidad de Zaragoza, C/Mariano Esquillor, s/n, 50018 Zaragoza, Spain; Instituto de Ciencia de Materiales de Aragón (ICMA), Universidad de Zaragoza-CSIC, C/Pedro Cerbuna 12, 50009 Zaragoza, Spain; Departamento de Química Física, Facultad de Ciencias, Universidad de Zaragoza, 50009, Zaragoza, Spain. Electronic address: pilarcea@unizar.es.

PMID: 32818926
DOI: 10.1016/j.colsurfb.2020.111315

Abstract

Isolated iron oxide magnetic nanoparticles (MNPs), 12 nm in diameter, coated with oleic acid molecules as capping agents have been deposited by the Langmuir-Blodgett (LB) method onto a model cell membrane incorporating 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and Cholesterol (Chol) in the 1:1 ratio, which was also fabricated by the LB technique. Atomic Force Microscopy (AFM) experiments showed that the application of an alternating magnetic field results in the embedding of the MNPs through the phospholipidic layer. These experimental results reveal that the heating of individual MNPs may induce a local increase in the fluidity of the film with a large control of the spatial and temporal specificity.

Keywords: Alternating magnetic Field; Langmuir-Blodgett films; Magnetic nanoparticles; Model cell membrane.

MeSH terms

Cell Membrane
Heating*
Magnetic Phenomena
Magnetics*
Microscopy, Atomic Force