Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

Methods Mol Biol. 2021:2210:113-121. doi: 10.1007/978-1-0716-0939-2_11.

Abstract

The type IX secretion system (T9SS) is the most recently discovered secretion system in the gram-negative bacteria and is specific to the Bacteroidetes phylum. It is comprised of at least 19 proteins, which together allows for the secretion and cell surface attachment of a specific group of proteins (T9SS substrates), that harbor a signal sequence at the C-terminus. Here we describe the structural characterization of the PorK, PorN and PorG components of the Porphyromonas gingivalis T9SS using electron microscopy and cross-linking mass spectrometry.

Keywords: Cross-linking; Electron microscopy; Gradient centrifugation; Mass spectrometry; PorG; PorK; PorN; Porphyromonas gingivalis; Protein complexes; Type IX secretion system (T9SS).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / genetics
  • Adhesins, Bacterial / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacterial Secretion Systems / genetics
  • Bacterial Secretion Systems / metabolism*
  • Mass Spectrometry / methods
  • Microscopy, Electron / methods
  • Porphyromonas gingivalis / genetics
  • Porphyromonas gingivalis / metabolism*
  • Protein Sorting Signals / genetics

Substances

  • Adhesins, Bacterial
  • Bacterial Proteins
  • Bacterial Secretion Systems
  • Protein Sorting Signals