Developing a Model for a siRNA Delivery System by Cancer Implantation into Zebrafish Circulation

Methods Mol Biol. 2021:2174:263-275. doi: 10.1007/978-1-0716-0759-6_17.

Abstract

In recent decades, zebrafish (Danio rerio) has become a major in vivo model for the evaluation of drug efficacies and toxicities. In the field of drug delivery research, zebrafish larvae are a suitable model for the use of fluorescent-labeled chemicals, nanoparticle, liposome, or micelle-mediated delivery systems because of their transparent body wall. In the current chapter, we describe the method to perform micelle-based siRNA delivery using cancer cells implanted into the circulation of zebrafish.

Keywords: EPR effect; Nanoamedicine; Xenotransplantation; Zebrafish; siRNA delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Carbocyanines / chemistry
  • Cell Line, Tumor
  • Cell Transplantation
  • Drug Delivery Systems / methods
  • Fluorescent Dyes / chemistry
  • Gene Transfer Techniques*
  • Humans
  • Larva / genetics
  • Luminescent Proteins / genetics
  • Melanoma / pathology
  • Micelles
  • Polylactic Acid-Polyglycolic Acid Copolymer / chemistry
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / pharmacology*
  • Red Fluorescent Protein
  • Transplants
  • Zebrafish* / genetics

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • Luminescent Proteins
  • Micelles
  • RNA, Small Interfering
  • cyanine dye 5
  • Polylactic Acid-Polyglycolic Acid Copolymer