Abstract
In recent decades, zebrafish (Danio rerio) has become a major in vivo model for the evaluation of drug efficacies and toxicities. In the field of drug delivery research, zebrafish larvae are a suitable model for the use of fluorescent-labeled chemicals, nanoparticle, liposome, or micelle-mediated delivery systems because of their transparent body wall. In the current chapter, we describe the method to perform micelle-based siRNA delivery using cancer cells implanted into the circulation of zebrafish.
Keywords:
EPR effect; Nanoamedicine; Xenotransplantation; Zebrafish; siRNA delivery.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Carbocyanines / chemistry
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Cell Line, Tumor
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Cell Transplantation
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Drug Delivery Systems / methods
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Fluorescent Dyes / chemistry
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Gene Transfer Techniques*
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Humans
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Larva / genetics
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Luminescent Proteins / genetics
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Melanoma / pathology
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Micelles
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Polylactic Acid-Polyglycolic Acid Copolymer / chemistry
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RNA, Small Interfering / chemistry
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RNA, Small Interfering / genetics
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RNA, Small Interfering / pharmacology*
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Red Fluorescent Protein
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Transplants
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Zebrafish* / genetics
Substances
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Carbocyanines
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Fluorescent Dyes
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Luminescent Proteins
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Micelles
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RNA, Small Interfering
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cyanine dye 5
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Polylactic Acid-Polyglycolic Acid Copolymer