Early diagnosis of diseases is of great importance because it increases the chance of a cure and significantly reduces treatment costs. Thus, development of rapid, sensitive, and reliable biosensing techniques is essential for the benefits of human life and health. As such, various nanomaterials have been explored to improve performance of biosensors, among which, carbon dots (CDs) have received enormous attention due to their excellent performance. In this Review, the recent advancements of CD-based biosensors have been carefully summarized. First, biosensors are classified according to their sensing strategies, and the role of CDs in these sensors is elaborated in detail. Next, several typical CD-based biosensors (including CD-only, enzymatic, antigen-antibody, and nucleic acid biosensors) and their applications are fully discussed. Last, advantages, challenges, and perspectives on the future trends of CD-based biosensors are highlighted.
Keywords: DNA; FRET; antibody; biosensor; carbon dots; enzyme; fluorescence quenching; graphene quantum dots; on−off−on; polymer dots.