Nanographene enfolded AuNPs sophisticatedly synchronized polycaprolactone based electrospun nanofibre scaffold for peripheral nerve regeneration

Richa Jaswal; Sita Shrestha; Bishnu Kumar Shrestha; Dinesh Kumar; Chan Hee Park; Cheol Sang Kim

doi:10.1016/j.msec.2020.111213

Nanographene enfolded AuNPs sophisticatedly synchronized polycaprolactone based electrospun nanofibre scaffold for peripheral nerve regeneration

Mater Sci Eng C Mater Biol Appl. 2020 Nov:116:111213. doi: 10.1016/j.msec.2020.111213. Epub 2020 Jun 20.

Authors

Richa Jaswal¹, Sita Shrestha², Bishnu Kumar Shrestha³, Dinesh Kumar⁴, Chan Hee Park⁵, Cheol Sang Kim⁶

Affiliations

¹ Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, South Korea; Division of Mechanical Design Engineering, Jeonbuk National University, Jeonju 561-756, South Korea.
² Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, South Korea.
³ Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, South Korea. Electronic address: bishnuaampipal@jbnu.ac.kr.
⁴ Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, South Korea. Electronic address: dineshkumar@jbnu.ac.kr.
⁵ Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, South Korea; Division of Mechanical Design Engineering, Jeonbuk National University, Jeonju 561-756, South Korea. Electronic address: biochan@jbnu.ac.kr.
⁶ Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, South Korea; Division of Mechanical Design Engineering, Jeonbuk National University, Jeonju 561-756, South Korea. Electronic address: chskim@jbnu.ac.kr.

PMID: 32806222
DOI: 10.1016/j.msec.2020.111213

Abstract

Herein, we report the bioactivity of monodispersed nanosized reduced graphene oxide (RGO) enfolded gold nanoparticles (AuNPs) engineered polycaprolactone (PCL) based electrospun composite scaffolds. The 2D patterns of PCL based nanofibers prepared by the homogenous distribution of RGO-AuNPs exhibited unique topological and biological features such as mechanical properties, porous structure, large surface area, high electrical conductivity, biodegradability, and resemble the natural extracellular matrix (ECM) that supports the adhesion, growth, proliferation, and differentiation of stem cells. The prepared composite nanofibers based scaffolds containing RGO-AuNPs accelerated neuronal cell functions and confirmed that the optimized concentration showed cytocompatibility to PC12 and S42 cells. The 0.0005 wt% loading of RGO-AuNPs on PCL has a huge impact on neurite growth which leads to an almost one-fold increase in neurite length growth. The present study provides a new strategic design of highly efficient scaffolds that have a significant direct impact on cell activity and could be a potential bioimplant for peripheral nerve repair.

Keywords: Cell viability; Gold nanoparticles; Nanofibre scaffold; Nanographene; Neuronal tissue engineering; PC12 cells.

MeSH terms

Cell Proliferation
Gold
Metal Nanoparticles*
Nanofibers*
Nerve Regeneration
Peripheral Nerves
Polyesters
Tissue Engineering
Tissue Scaffolds

Substances

Polyesters
polycaprolactone
Gold