Bifidobacterium canis sp. nov., a novel member of the Bifidobacterium pseudolongum phylogenetic group isolated from faeces of a dog (Canis lupus f. familiaris)

Abstract

A fructose-6-phosphate phosphoketolase-positive strain (GSD1FS^T) was isolated from a faecal sample of a 3 weeks old German Shepherd dog. The closest related taxa to isolate GSD1FS^T based on results from the EZBioCloud database were Bifidobacterium animalis subsp. animalis ATCC 25527^T, Bifidobacterium animalis subsp. lactis DSM 10140^T and Bifidobacterium anseris LMG 30189^T, belonging to the Bifidobacterium pseudolongum phylogenetic group. The resulting 16S rRNA gene identities (compared length of 1454 nucleotides) towards these taxa were 97.30, 97.23 and 97.09 %, respectively. The pairwise similarities of strain GSD1FS^T using argS, atpA, fusA, hsp60, pyrG, rpsC, thrS and xfp gene fragments to all valid representatives of the B. pseudolongum phylogenetic group were in the concatenated range of 83.08-88.34 %. Phylogenomic analysis based on whole-genome methods such as average nucleotide identity revealed that bifidobacterial strain GSD1FS^T exhibits close phylogenetic relatedness (88.17 %) to Bifidobacetrium cuniculi LMG 10738^T. Genotypic characteristics and phylogenetic analyses based on nine molecular markers, as well as genomic and comparative phenotypic analyses, clearly proved that the evaluated strain should be considered as representing a novel species within the B. pseudolongum phylogenetic group named as Bifidobacterium canis sp. nov. (GSD1FS^T=DSM 105923^T=LMG 30345^T=CCM 8806^T).

Keywords: Bifidobacterium; Bifidobacterium pseudolongum phylogenetic group; MALDI-TOF MS; dogs.