The evolving capabilities of enzyme-mediated proximity labeling

Ying Zhou; Peng Zou

doi:10.1016/j.cbpa.2020.06.013

The evolving capabilities of enzyme-mediated proximity labeling

Curr Opin Chem Biol. 2021 Feb:60:30-38. doi: 10.1016/j.cbpa.2020.06.013. Epub 2020 Aug 12.

Authors

Ying Zhou¹, Peng Zou²

Affiliations

¹ College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871, China.
² College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871, China; Peking-Tsinghua Center for Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing, 100871, China. Electronic address: zoupeng@pku.edu.cn.

PMID: 32801087
DOI: 10.1016/j.cbpa.2020.06.013

Abstract

The subcellular organization of proteins and RNA molecules is crucial for their proper functions. Over the past decade, both ligase-mediated and peroxidase-mediated proximity labeling (PL) techniques have been developed to map biomolecules at near-nanometer spatial resolution and subminute temporal resolution. These methods are shedding light on the spatial arrangement of proteome and transcriptome in their native context. Here, we review the recent evolution and applications of PL techniques, compare and contrast the two classes of methods, and highlight emerging trends and future opportunities.

Keywords: APEX; BioID; Biotin ligase; Peroxidase; Proximity labeling.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Enzymes / metabolism*
Staining and Labeling / methods*

Substances

Enzymes