Overproduction of plant nuclear export signals enhances diamide tolerance in Schizosaccharomyces pombe

Xiaoling Ma; Xing Huang; Zhengli Jiao; Lilong He; Yongqing Li; David W Ow

doi:10.1016/j.bbrc.2020.07.054

Overproduction of plant nuclear export signals enhances diamide tolerance in Schizosaccharomyces pombe

Biochem Biophys Res Commun. 2020 Oct 20;531(3):335-340. doi: 10.1016/j.bbrc.2020.07.054. Epub 2020 Aug 13.

Authors

Xiaoling Ma¹, Xing Huang¹, Zhengli Jiao¹, Lilong He¹, Yongqing Li², David W Ow³

Affiliations

¹ Plant Gene Engineering Center, Chinese Academy of Sciences Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China.
² Plant Gene Engineering Center, Chinese Academy of Sciences Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
³ Plant Gene Engineering Center, Chinese Academy of Sciences Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China. Electronic address: dow@berkeley.edu.

PMID: 32800339
DOI: 10.1016/j.bbrc.2020.07.054

Abstract

The nuclear export signal (NES) endows a protein nuclear export ability. Surprisingly, our previous study shows that just the NES peptide of Schizosaccharomyces pombe Oxs1 (SpOxs1^NES) can confer diamide tolerance by competing with transcription factor Pap1 for nuclear transport. This finding intrigued us to test the function of NESs from heterologous organisms. The Arabidopsis thaliana zinc finger transcription factor OXIDATIVE STRESS 2 (AtOXS2) is a nucleocytoplasmic shuttling protein and nearly all OXS2 members from maize and rice contain an NES. In this study, we find that the plant OXS2 members and their C-terminus (AT3 peptide) can confer diamide tolerance due to their NESs, and amino acids in non-conserved as well as conserved positions are necessary for the diamide tolerance. As in SpOxs1^NES, the enhanced tolerance to diamide in fission yeast depends on Pap1. Like SpOxs1^NES, OXS2 family NESs appear to compete for nuclear transport of the Pap1-like Arabidopsis protein bZIP10, as when overproduced in Arabidopsis protoplasts, bZIP10 is retained in the nucleus.

Keywords: Nuclear export signal; OXS2 family; Pap1; bZIP10.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptation, Physiological
Amino Acid Sequence
Amino Acid Substitution
Cell Nucleus / metabolism
Conserved Sequence
Diamide / metabolism*
Nuclear Export Signals*
Peptides / metabolism
Plant Proteins / chemistry*
Plant Proteins / metabolism*
Schizosaccharomyces / metabolism*
Subcellular Fractions / metabolism

Substances

Nuclear Export Signals
Peptides
Plant Proteins
Diamide