Heterogeneous expression of plasma-membrane-localised OsOSCA1.4 complements osmotic sensing based on hyperosmolality and salt stress in Arabidopsis osca1 mutant

Yuanjun Zhai; Zhaohong Wen; Yang Han; Wenqing Zhuo; Fang Wang; Chao Xi; Jin Liu; Ping Gao; Heping Zhao; Yingdian Wang; Youjun Wang; Shengcheng Han

doi:10.1016/j.ceca.2020.102261

Heterogeneous expression of plasma-membrane-localised OsOSCA1.4 complements osmotic sensing based on hyperosmolality and salt stress in Arabidopsis osca1 mutant

Cell Calcium. 2020 Nov:91:102261. doi: 10.1016/j.ceca.2020.102261. Epub 2020 Aug 8.

Authors

Yuanjun Zhai¹, Zhaohong Wen², Yang Han³, Wenqing Zhuo⁴, Fang Wang⁵, Chao Xi⁶, Jin Liu⁷, Ping Gao⁸, Heping Zhao⁹, Yingdian Wang¹⁰, Youjun Wang¹¹, Shengcheng Han¹²

Affiliations

¹ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: 821541850@qq.com.
² Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: wen.zhaohong@163.com.
³ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: 489616221@qq.com.
⁴ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: 423468956@qq.com.
⁵ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: 501910625@qq.com.
⁶ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: xichao@bnu.edu.cn.
⁷ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: liujin@bnu.edu.cn.
⁸ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: gaoping1008@bnu.edu.cn.
⁹ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: hpzhao@bnu.edu.cn.
¹⁰ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: ydwang@bnu.edu.cn.
¹¹ Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: wyoujun@bnu.edu.cn.
¹² Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: schan@bnu.edu.cn.

PMID: 32798853
DOI: 10.1016/j.ceca.2020.102261

Abstract

In plants, both hyperosmolality and salt stress induce cytosolic calcium increases within seconds, referred to as the hyperosmolality-induced [Ca²⁺]_cyt increases, OICI_cyt, and salt stress-induced [Ca²⁺]_cyt increases, SICI_cyt. Previous studies have shown that Arabidopsis reduced hyperosmolality-induced [Ca²⁺]_i increase 1 (OSCA1.1) encodes a hyperosmolality-gated calcium-permeable channel that mediates OICI_cyt in guard cells and root cells. Multiple OSCA members exist in plants; for example, Oryza sativa has 11 OsOSCAs genes, indicating that OSCAs have diverse biological functions. Here, except for OsOSCA4.1, ten full-length OsOSCAs were separately subcloned, in which OsOSCA1.4 was exclusively localised to the plasma membrane and other nine OsOSCAs-eYFP co-localised with an endoplasmic reticulum marker in Arabidopsis mesophyll protoplasts. OsOSCA1.4 was further identified as a calcium-permeable ion channel that activates an inward current after receiving an osmotic signal exerted by hyperosmolality or salt stress, and mediates OICI_cyt and SICI_cyt in human embryonic kidney 293 (HEK293) cells. Moreover, overexpression of OsOSCA1.4 in Arabidopsis osca1 mutant complemented osmotic Ca²⁺ signalling, root growth, and stomatal movement in response to hyperosmolality and salt stress. These results will facilitate further study of OsOSCA-mediated calcium signalling and its distinct roles in rice growth and development.

Keywords: Calcium-permeable ion channel; Functional complementation; OICI(cyt); OsOSCA1.4; SICI(cyt); osca1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / physiology*
Cell Membrane / metabolism*
Cytosol / metabolism
Endoplasmic Reticulum / metabolism
HEK293 Cells
Humans
Mesophyll Cells / metabolism
Mutation / genetics*
Oryza / metabolism*
Osmolar Concentration*
Osmosis*
Phenotype
Plant Proteins / metabolism*
Plants, Genetically Modified
Protoplasts / metabolism
Salt Stress / physiology*
Subcellular Fractions / metabolism

Substances

Plant Proteins