Regulatory functions of gga-miR-218 in spermatogonial stem cells meiosis by targeting Stra8

Yingjie Wang; Lei Zhang; Wenhui Zhang; Changhua Sun; Zheyu Deng; Cai Hu; Ahmed Kamel Elsayed; Xinqi Zhou; Tingting Li; Qisheng Zuo; Xinglong Wang; Bichun Li; Ya-Ni Zhang

doi:10.1016/j.mod.2020.103636

Regulatory functions of gga-miR-218 in spermatogonial stem cells meiosis by targeting Stra8

Mech Dev. 2020 Dec:164:103636. doi: 10.1016/j.mod.2020.103636. Epub 2020 Aug 13.

Authors

Affiliations

¹ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu province, PR China; College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu province, PR China; Key Laboratory for Animal Genetics, Breeding, Reproduction, and Molecular Design of Jiangsu Province, Yangzhou 225009, Jiangsu province, PR China.
² Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, Jiangsu province, PR China.
³ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu province, PR China; Key Laboratory for Animal Genetics, Breeding, Reproduction, and Molecular Design of Jiangsu Province, Yangzhou 225009, Jiangsu province, PR China.
⁴ Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt.
⁵ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu province, PR China; Key Laboratory for Animal Genetics, Breeding, Reproduction, and Molecular Design of Jiangsu Province, Yangzhou 225009, Jiangsu province, PR China. Electronic address: ynzhang@yzu.edu.cn.

PMID: 32798699
DOI: 10.1016/j.mod.2020.103636

Abstract

MicroRNAs play a crucial role in sperm formation, but its specific function remains unknown. Here, we found that gga-miR-218 regulates chicken sperm formation through in/ex vivo experiments. We constructed over-expression/interference carrier to overexpress and inhibit gga-miR-218 in chicken spermatogonial stem cells, separately, the detection of haploid and QRT-PCR of meiosis related genes revealed that gga-miR-218 inhibits meiosis. After injection of miR-218 in vivo, semen concentration and HE (Hematoxylin and Eosin staining) revealed that gga-miR-218 inhibits meiosis. Meanwhile, we discovered that gga-miR-218 could target Stra8 by prediction software which can inhibit the wild-type fluorescence activity by co-transfection of gga-miR-218 with the Stra8 3' untranslated regions fluorescent reporter vector (wild-type/mutant), QRT-PCR and Western blot showed that gga-miR-218 inhibits the expression level of Stra8 by targeting its 3' untranslated regions directly. Finally, we suggest that gga-miR-218 could target to srta8 directly and inhibit spermatogenesis.

Keywords: Meiosis; Spermatogonial stem cells; Stra8; gga-miR-218.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
Adaptor Proteins, Signal Transducing / genetics
Animals
Avian Proteins / genetics
Chickens / genetics*
Gene Expression
Meiosis*
MicroRNAs / genetics*
Spermatogenesis*
Stem Cells / cytology*

Substances

3' Untranslated Regions
Adaptor Proteins, Signal Transducing
Avian Proteins
MicroRNAs