Development of luminol-fluorescamine-PVP chemiluminescence system and its application to sensitive tyrosinase determination

Chao Wang; Yequan Chen; Dmytro Snizhko; Fangxin Du; Xiangui Ma; Baohua Lou; Jianping Li; Guobao Xu

doi:10.1016/j.talanta.2020.121177

Development of luminol-fluorescamine-PVP chemiluminescence system and its application to sensitive tyrosinase determination

Talanta. 2020 Oct 1:218:121177. doi: 10.1016/j.talanta.2020.121177. Epub 2020 May 21.

Authors

Chao Wang¹, Yequan Chen¹, Dmytro Snizhko², Fangxin Du³, Xiangui Ma³, Baohua Lou⁴, Jianping Li⁵, Guobao Xu⁶

Affiliations

¹ College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; Laboratory of Analytical Optochemotronics, Kharkiv National University of Radio Electronics, 14 Nauki Ave., Kharkiv, 61066, Ukraine.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Science and Technology of China, Hefei, 230026, China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Science and Technology of China, Hefei, 230026, China. Electronic address: loubh@ciac.ac.cn.
⁵ College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China. Electronic address: likianping@263.net.
⁶ College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; Laboratory of Analytical Optochemotronics, Kharkiv National University of Radio Electronics, 14 Nauki Ave., Kharkiv, 61066, Ukraine. Electronic address: guobaoxu@ciac.ac.cn.

PMID: 32797925
DOI: 10.1016/j.talanta.2020.121177

Abstract

Fluorescamine is a popular fluorescent probe. We report for the first time that luminol chemiluminescence (CL) can be enhanced by fluorescamine in the presence of PVP. The CL intensity of luminol-fluorescamine-PVP is about 26 times stronger than that of luminol. Both the removal of oxygen and the addition of superoxide dismutase (SOD) decrease CL intensity, thiourea and NaN₃ have little effect on CL intensities, indicating that O₂^•- is critical for CL. Interestingly, o-quinone generated from phenol by tyrosinase obviously inhibited the CL intensity. Inspired by such quenching effect on the luminol-fluorescamine-PVP CL system, a sensitive CL sensing for the determination of tyrosinase activity was developed. The method can detect tyrosinase in the range of 0.07-1.5 μg mL^-1 (0.19-4.02 U mL^-1) with the detection limit of 0.035 μg mL^-1 (0.094 U mL^-1). Moreover, this method exhibits satisfied recoveries for the spiked human serum samples.

Keywords: Fluorescamine; Luminol chemiluminescence; Phenol; Polyvinylpyrrolidone; Tyrosinase activity.