N,N-dicarboxymethyl Perylene-diimide modified CeCoO3: Enhanced peroxidase activity, synergetic catalytic mechanism and glutathione colorimetric sensing

Xiaohan Chen; Shuai Han; Ning Li; Jiajia Lian; Yuexing Zhang; Qingyun Liu; Xianxi Zhang; Xiao Zhang

doi:10.1016/j.talanta.2020.121142

N,N-dicarboxymethyl Perylene-diimide modified CeCoO₃: Enhanced peroxidase activity, synergetic catalytic mechanism and glutathione colorimetric sensing

Talanta. 2020 Oct 1:218:121142. doi: 10.1016/j.talanta.2020.121142. Epub 2020 May 23.

Authors

Xiaohan Chen¹, Shuai Han¹, Ning Li¹, Jiajia Lian¹, Yuexing Zhang², Qingyun Liu³, Xianxi Zhang⁴, Xiao Zhang⁵

Affiliations

¹ College of Chemical and Biological Engineering, State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, PR China.
² Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China.
³ College of Chemical and Biological Engineering, State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, PR China. Electronic address: qyliu@sdust.edu.cn.
⁴ Shandong Provincial Key Laboratory/Collaborative Innovation Center of Chemical Energy Storage & Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, PR China.
⁵ Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.

PMID: 32797899
DOI: 10.1016/j.talanta.2020.121142

Abstract

N,N-dicarboxymethyl Perylene-diimide (PDI) modified CeCoO₃ nanocomposites were prepared by a two-step method. After modification with PDI molecules, the obtained PDI-CeCoO₃ nanocomposites were demonstrated to possess the heightened peroxidase-like activity, compared with that of pure CeCoO₃ nanoparticles. In the presence of H₂O₂, the heightened peroxidase-like behaviors of PDI-CeCoO₃ were evaluated by the oxidation of the colorless substrate 3,3,5,5-tetramethylbenzidine (TMB) into blue oxTMB, which was detected visually only in 4 min. Importantly, a systematic study of catalytic activity of PDI-CeCoO₃ by different means, including fluorescent probe, electrochemical data, diffuse reflection spectra together with free radical scavenger is executed, verifying that the catalytic activity were from O₂^- and electron holes (h⁺). And, the transfer of photogenerated carriers in the PDI-CeCoO₃ was the Z-scheme heterojuntion mechanism. Furthermore, the peroxidase-like activity of PDI-CeCoO₃ was significantly inhibited by Glutathione (GSH), resulting in fading of blue oxTMB. Based on this, a colorimetric assay for GSH biosensing has been developed. And, the liner range for GSH detection is from 1 to 10 μM with a detection limit of 0.658 μM. The recovery of GSH with different concentrations from 90.0% to 105.9% and the relative standard deviation (RSD) from 1.9% to 5.1%. This colorimetric sensor can be used to detect GSH in real samples.

Keywords: Biosensing; CeCoO(3); Colorimetric; Glutathione; Peroxidase.