Integrated Metabolic and Epigenomic Reprograming by H3K27M Mutations in Diffuse Intrinsic Pontine Gliomas

Chan Chung; Stefan R Sweha; Drew Pratt; Benita Tamrazi; Pooja Panwalkar; Adam Banda; Jill Bayliss; Debra Hawes; Fusheng Yang; Ho-Joon Lee; Mengrou Shan; Marcin Cieslik; Tingting Qin; Christian K Werner; Daniel R Wahl; Costas A Lyssiotis; Zhiguo Bian; J Brad Shotwell; Viveka Nand Yadav; Carl Koschmann; Arul M Chinnaiyan; Stefan Blüml; Alexander R Judkins; Sriram Venneti

doi:10.1016/j.ccell.2020.07.008

Integrated Metabolic and Epigenomic Reprograming by H3K27M Mutations in Diffuse Intrinsic Pontine Gliomas

Cancer Cell. 2020 Sep 14;38(3):334-349.e9. doi: 10.1016/j.ccell.2020.07.008. Epub 2020 Aug 13.

Authors

Chan Chung¹, Stefan R Sweha², Drew Pratt¹, Benita Tamrazi³, Pooja Panwalkar¹, Adam Banda¹, Jill Bayliss¹, Debra Hawes⁴, Fusheng Yang⁴, Ho-Joon Lee⁵, Mengrou Shan⁵, Marcin Cieslik⁶, Tingting Qin⁷, Christian K Werner⁸, Daniel R Wahl⁸, Costas A Lyssiotis⁵, Zhiguo Bian⁹, J Brad Shotwell⁹, Viveka Nand Yadav¹⁰, Carl Koschmann¹⁰, Arul M Chinnaiyan⁶, Stefan Blüml³, Alexander R Judkins⁴, Sriram Venneti¹¹

Affiliations

¹ Laboratory of Brain Tumor Metabolism and Epigenetics, Department of Pathology, University of Michigan Medical School, Michigan Medicine, University of Michigan, 3520E MSRB 1, 1150 West Medical Center Drive, Ann Arbor, MI 48109-41804, USA; Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
² Laboratory of Brain Tumor Metabolism and Epigenetics, Department of Pathology, University of Michigan Medical School, Michigan Medicine, University of Michigan, 3520E MSRB 1, 1150 West Medical Center Drive, Ann Arbor, MI 48109-41804, USA; Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
³ Department of Radiology, Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
⁴ Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
⁵ Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
⁶ Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Michigan Center for Translational Pathology, Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109, USA.
⁷ Department of Computational Medicine and Bioinformatics, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
⁸ Department of Radiation Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
⁹ Centralized Medicinal Chemistry, AbbVie, 1 North Waukegan Road, North Chicago, IL 60064, USA.
¹⁰ Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
¹¹ Laboratory of Brain Tumor Metabolism and Epigenetics, Department of Pathology, University of Michigan Medical School, Michigan Medicine, University of Michigan, 3520E MSRB 1, 1150 West Medical Center Drive, Ann Arbor, MI 48109-41804, USA; Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Electronic address: svenneti@med.umich.edu.

Abstract

H3K27M diffuse intrinsic pontine gliomas (DIPGs) are fatal and lack treatments. They mainly harbor H3.3K27M mutations resulting in H3K27me3 reduction. Integrated analysis in H3.3K27M cells, tumors, and in vivo imaging in patients showed enhanced glycolysis, glutaminolysis, and tricarboxylic acid cycle metabolism with high alpha-ketoglutarate (α-KG) production. Glucose and/or glutamine-derived α-KG maintained low H3K27me3 in H3.3K27M cells, and inhibition of key enzymes in glycolysis or glutaminolysis increased H3K27me3, altered chromatin accessibility, and prolonged survival in animal models. Previous studies have shown that mutant isocitrate-dehydrogenase (mIDH)1/2 glioma cells convert α-KG to D-2-hydroxyglutarate (D-2HG) to increase H3K27me3. Here, we show that H3K27M and IDH1 mutations are mutually exclusive and experimentally synthetic lethal. Overall, we demonstrate that H3.3K27M and mIDH1 hijack a conserved and critical metabolic pathway in opposing ways to maintain their preferred epigenetic state. Consequently, interruption of this metabolic/epigenetic pathway showed potent efficacy in preclinical models, suggesting key therapeutic targets for much needed treatments.

Keywords: D-2HG; DIPG; H3K27me3; IDH mutation; epigenetics; glutaminolysis; glycolysis; histone methylation; histone mutation; metabolism; α-KG.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Stem Neoplasms / genetics*
Brain Stem Neoplasms / metabolism
Cell Line, Tumor
Diffuse Intrinsic Pontine Glioma / genetics*
Diffuse Intrinsic Pontine Glioma / metabolism
Epigenomics / methods*
Gene Expression Regulation, Neoplastic
Glycolysis
Histones / genetics*
Histones / metabolism
Humans
Lysine / genetics
Lysine / metabolism
Methylation
Mice, Inbred NOD
Mice, Knockout
Mice, Nude
Mice, SCID
Mutation*
Transplantation, Heterologous

Substances

Histones
Lysine

Abstract

Publication types

MeSH terms

Substances

Grants and funding