Effects of lysine and arginine on the properties of low-salt mince gel from striped catfish (Pangasianodon hypophthalmus)

J Food Sci. 2020 Sep;85(9):2681-2687. doi: 10.1111/1750-3841.15368. Epub 2020 Aug 14.

Abstract

Effects of basic amino acids, lysine (Lys) and arginine (Arg), at different levels (0%, 0.5%, and 1%, based on mince weight) on properties of striped catfish (Pangasianodon hypophthalmus) mince gel containing low salt (LS) and high salt (HS) were investigated. Without Lys or Arg addition, HS gel had the higher textural properties including hardness, chewiness, as well as cohesiveness than LS gel (P < 0.05) and the highest values were achieved when 1% Arg was incorporated in both LS and HS gels (P < 0.05). Arg had no effect on acceptability of mince gel. However, whiteness was decreased in HS gel when Arg was applied. Autolysis of gel was lower in HS gel containing Arg. No differences in protein patterns among all gel samples were found. Addition of Arg could increase the gelling ability of both LS and HS mince during heating as evidenced by higher storage modulus (G') and viscous modulus (G″). Mince gel added with Arg had orderly interconnected structure and their microstructure was finer than that without Arg. Therefore, Arg could be used in LS gel from striped catfish mince, in which quality of gel was equivalent to HS counterpart. PRACTICAL APPLICATION: Basic amino acid, especially arginine (Arg) with guanidinium group, could increase repulsive force between protein molecules at low-salt concentration (0.5%). This resulted in high solubilization of muscle proteins, whereas gel formation or gel strength was higher than that containing high salt (2.5% to 3.5%). Thus, Arg could be used for production of fish mince gel containing low salt with lowered health risk.

Keywords: Pangas; arginine; gelation; low salt; lysine; mince; striped catfish.

MeSH terms

  • Animals
  • Arginine / analysis*
  • Catfishes
  • Fish Products / analysis*
  • Fish Proteins / chemistry
  • Gels / chemistry
  • Hardness
  • Lysine / analysis*
  • Muscle Proteins / chemistry
  • Sodium Chloride / analysis

Substances

  • Fish Proteins
  • Gels
  • Muscle Proteins
  • Sodium Chloride
  • Arginine
  • Lysine