Enzyme-labeled type III bacterial Fc receptors. A versatile tracer for immunoassay

J Immunol Methods. 1988 Mar 16;107(2):273-80. doi: 10.1016/0022-1759(88)90228-1.

Abstract

The type III bacterial Fc receptor isolated form a group C streptococcus has been conjugated to alkaline phosphatase and used as a tracer in a variety of direct and indirect immunoassays. These immunoassays have utilized specific antibodies prepared in species whose immunoglobulins are poorly reactive with the type I Fc receptor, staphylococcal protein A. The value of the type III Fc receptor as a tracer for immunoassays utilizing antibodies produced in sheep and goats is documented.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkaline Phosphatase
  • Animals
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin G
  • Immunoglobulins / analysis*
  • Plasminogen / analysis*
  • Receptors, Fc / immunology*
  • Sheep
  • Staphylococcal Protein A / immunology*
  • Streptococcus / immunology*
  • Streptokinase / analysis

Substances

  • Immunoglobulin G
  • Immunoglobulins
  • Receptors, Fc
  • Staphylococcal Protein A
  • Plasminogen
  • Alkaline Phosphatase
  • Streptokinase