Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body

Mst Nushrat Yiasmin; Md Serajul Islam; He He; Yaxian Liu; Mingming Wang; Ruijin Yang; Xiao Hua

doi:10.1016/j.ijbiomac.2020.08.037

Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body

Int J Biol Macromol. 2020 Dec 1:164:1879-1888. doi: 10.1016/j.ijbiomac.2020.08.037. Epub 2020 Aug 10.

Authors

Mst Nushrat Yiasmin¹, Md Serajul Islam², He He³, Yaxian Liu³, Mingming Wang⁴, Ruijin Yang³, Xiao Hua⁵

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China.
⁴ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
⁵ State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address: huaxiao@jiangnan.edu.cn.

PMID: 32791276
DOI: 10.1016/j.ijbiomac.2020.08.037

Abstract

The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order. Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps. Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR). GC-MS and methylation analysis that linkages were mainly β-1,3 and β-1,6, β-1,4 and β-1,2 bonds in WIP. Seven main oligomer products were detected and their structures characterized by mass spectrum. Experimental results shown the similarity in structure between water soluble polysaccharides (WSP) and water insoluble polysaccharides (WIP), thus WSP can be the product of cell wall by breakdown.

Keywords: Polysaccharides; Purification; Structure characterization.

MeSH terms

Chromatography, Gel / methods
Chromatography, Ion Exchange / methods
Fruiting Bodies, Fungal / chemistry
Fungal Polysaccharides / chemistry
Grifola / chemistry*
Grifola / isolation & purification*
Magnetic Resonance Spectroscopy / methods
Polysaccharides / chemistry
Polysaccharides / isolation & purification*
Water / analysis

Substances

Fungal Polysaccharides
Polysaccharides
Water