Automated diatom searching in the digital scanning electron microscopy images of drowning cases using the deep neural networks

Int J Legal Med. 2021 Mar;135(2):497-508. doi: 10.1007/s00414-020-02392-z. Epub 2020 Aug 13.

Abstract

Forensic diatom test has been widely accepted as a way of providing supportive evidences in the diagnosis of drowning. The current workflow is primarily based on the observation of diatoms by forensic pathologists under a microscopy, and this process can be very time-consuming. In this paper, we demonstrate a deep learning-based approach for automatically searching diatoms in scanning electron microscopic images. Cross-validation studies were performed to evaluate the influence of magnification on performance. Moreover, various training strategies were tested to improve the performance of detection. The conclusion shows that our approach can satisfy the necessary requirements to be integrated as part of an automatic forensic diatom test.

Keywords: Artificial intelligence; Diatom test; Forensic science; Object detection; Scanning electron microscopy.

MeSH terms

  • Deep Learning*
  • Diatoms / classification
  • Diatoms / isolation & purification*
  • Drowning / diagnosis*
  • Forensic Pathology / methods*
  • Humans
  • Microscopy, Electron, Scanning
  • Neural Networks, Computer*