Fabrication of curcumin-zein-ethyl cellulose composite nanoparticles using antisolvent co-precipitation method

Abstract

The stable colloidal nano-dispersion of curcumin (CU) loaded zein-ethyl cellulose (ZN-EC) as three hydrophobic agent in water was prepared using two step antisolvent co-precipitation method. The EC coated NPs were prepared by adding EC in ethyl acetate to the ZN-CU NPs at a concentration ratio of 1: 3.5 w/v. The prepared colloidal suspension of ZN-EC showed high physical stability during storage time. The particle diameters and zeta potential values of ZN-CU and ZN-CU-EC colloidal suspensions were 140 ± 12 nm, 38 ± 2 mV and 179 ± 12 nm, 12 ± 2 mV, respectively. Based on Scanning electron microscopy (SEM) images, participation of EC on the surface of ZN-CU particles could reduce the sticky appearance of particles. Encapsulation efficiency of CU in NPs did not improve after precipitation of EC, but the stability of NPs against pH changes, increased and release rate of CU from NPs at different pH values (3-8) were significantly reduced in comparison of ZN-CU NPs. The EC coated NPs showed the excessive protection for CU antioxidant activity during storage. In conclusion, the prepared NPs, with high physical stability, have good potential for encapsulation and delivery of CU to colon region.

Keywords: Anti-solvent; Curcumin; Ethyl cellulose; Zein.