Complex gene regulation between young and old soybean leaves in responses to manganese toxicity

Ying Liu; Yingbin Xue; Baoxing Xie; Shengnan Zhu; Xing Lu; Cuiyue Liang; Jiang Tian

doi:10.1016/j.plaphy.2020.07.002

Complex gene regulation between young and old soybean leaves in responses to manganese toxicity

Plant Physiol Biochem. 2020 Oct:155:231-242. doi: 10.1016/j.plaphy.2020.07.002. Epub 2020 Aug 1.

Authors

Ying Liu¹, Yingbin Xue², Baoxing Xie³, Shengnan Zhu³, Xing Lu³, Cuiyue Liang⁴, Jiang Tian⁵

Affiliations

¹ Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, PR China; Department of Biotechnology, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, PR China.
² Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, PR China; Department of Resources and Environmental Sciences, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, PR China.
³ Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, PR China.
⁴ Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, PR China. Electronic address: liangcy@scau.edu.cn.
⁵ Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, PR China. Electronic address: jtian@scau.edu.cn.

PMID: 32781273
DOI: 10.1016/j.plaphy.2020.07.002

Abstract

Manganese (Mn) is an essential micronutrient for plant growth. However, excess manganese is toxic and inhibits crop production. Although it is widely known that physiological and molecular mechanisms underlie plant responses to Mn toxicity, few studies have been conducted to compare Mn tolerance capabilities between young and old leaves in plants; thus, the mechanisms underlying Mn tolerance in different plant tissues or organs are not fully understood. In this study, the dose responses of soybean to Mn availability were investigated. Genome-wide transcriptomic analysis was subsequently conducted to identify the differentially expressed genes (DEGs) in both young and old leaves of soybean in responses to Mn toxicity. Our results showed that excess Mn severely inhibited soybean growth and increased both Mn accumulation in and brown spots on soybean leaves, especially for the old leaves, strongly suggesting that more Mn was allocated to old leaves in soybean. Transcriptomic profiling revealed that totals of 4410 and 2258 DEGs were separately identified in young leaves and old leaves. Furthermore, only 944 DEGs were found to be commonly regulated in both young and old leaves of soybean, strongly suggesting distinct responses present in soybean young and old leaves in responses to Mn toxicity.

Keywords: Genome-wide transcriptomic analysis; Leaves; Mn toxicity; Soybean.

MeSH terms

Gene Expression Regulation, Plant*
Glycine max / drug effects
Glycine max / genetics*
Manganese / toxicity*
Plant Leaves / drug effects
Transcriptome

Substances

Manganese