An improved structure-switch aptamer-based fluorescent Pb2+ biosensor utilizing the binding induced quenching of AMT to G-quadruplex

Fenghua Geng; Dandan Wang; Li Feng; Guixin Li; Maotian Xu

doi:10.1039/d0cc03669b

An improved structure-switch aptamer-based fluorescent Pb²⁺ biosensor utilizing the binding induced quenching of AMT to G-quadruplex

Chem Commun (Camb). 2020 Sep 16;56(72):10517-10520. doi: 10.1039/d0cc03669b. Epub 2020 Aug 11.

Authors

Fenghua Geng¹, Dandan Wang, Li Feng, Guixin Li, Maotian Xu

Affiliation

¹ National Engineering Research Center of Coal Preparation and Purification, Key Laboratory of Coal Processing and Efficient Utilization of Ministry of Education, School of Chemical Engineering and Technology, China University of Mining & Technology, Xuzhou, 221116, China. 8422127@alu.fudan.edu.cn.

PMID: 32779681
DOI: 10.1039/d0cc03669b

Abstract

An improved aptamer-based fluorescent Pb²⁺ biosensor utilizing the binding induced quenching of AMT to G-quadruplex has been rationally designed with a LOD of 3.6 nM. The utility of the developed biosensor was demonstrated by the successful detection of Pb²⁺ in real complex clinical samples with satisfactory recovery and good reproducibility.

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques / methods*
Fluorescent Dyes / chemistry*
G-Quadruplexes*
Humans
Lead / analysis*
Lead / chemistry*
Limit of Detection
Reproducibility of Results
Spectrometry, Fluorescence

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Lead