Utilization of biosolids for glucose oxidase production: A potential bio-fenton reagent for advanced oxidation process for removal of pharmaceutically active compounds

Abstract

The current work focuses on the production of glucose oxidase (GOD) in sterilized biosolid (BS) slurries containing BS and municipal wastewater effluent. Various parameters were optimized for maximizing the GOD production and the effects of biostimulation on GOD production was investigated by adding synthetic media components. The studies on inoculum characteristics at an inoculum age of 72 h and inoculum size of 20% (w/v) produced high GOD activities of around 6012 U/L in 25% (dw/v) BS media. Further, the effect of ultrasonication time was determined to release BS-bound GOD in order to maximize enzymes recovery. Using 1000 U/L of the BS-based GOD for 0.55 M glucose oxidation produced the maximum H₂O₂ concentration of 216 ppm. The produced H₂O₂ was utilized for bio-Fenton based advanced oxidation process for the partial removal of 15 pharmaceutically active compounds.

Keywords: Bio-fenton; Biosolids; Glucose oxidase; Pharmaceutically active compounds; Valorization.