This work is aimed to develop of a new class of versatile aptasensor to specifically detect aflatoxin B1 (AFB1) using dual-channel detection method. To achieve this objective, gold nanoparticles (AuNPs) having peroxidase-like activity and capability of promoting silver deposition were used as the versatile label for both colorimetric and electrochemical techniques. First of all, aptamer (apt) modified Fe3O4@Au magnetic beads (MBs-apt) and cDNA modified AuNPs (cDNA-AuNPs) were prepared to use as capture probes and signal probes, respectively. Taking advantage of hybridization reaction between apt and cDNA, these two probes were coupled with each other to generate MBs-apt/cDNA-AuNPs bioconjugations. The high affinity between apt and AFB1 made cDNA-AuNPs detached from MBs-apt, and the released signal probes were separated and collected using an external magnetic field and used for both colorimetric and electrochemical detection channels. The dual-channel signals were directly proportional to logarithm of AFB1 concentration within the ranges of 5-200 ng mL-1 and 0.05-100 ng mL-1. The detection limit can reach as low as 35 pg mL-1 and 0.43 pg mL-1 for colorimetric and electrochemical channel, respectively. Moreover, the proposed aptasensor has been successfully applied to determine AFB1 in corn samples with satisfactory results. This dual-channel detection method can not only improve the detection precision and diversity significantly, but also can reduce the false-negative and-positive rates in food quality monitoring. We believe we have provided a general strategy with the convincing dual-readout mode which possess great promising in all of the aptamer related fields.
Keywords: Aflatoxin B1; Aptamer; Aptasensor; Dual-channel detection; Gold nanoparticles.
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