Comparative data analsysis of two multi-drug resistant homoserine lactone and rhamnolipid producing Pseudomonas aeruginosa from diabetic foot infected patient

Prakhar Srivastava; Sankaranarayanan Gomathinayagam; Nalini Easwaran; Gowri Sankar; E Padmavathi; Manoharan Shankar; Kodiveri M Gothandam; Karthikeyan Sivashanmugam

doi:10.1016/j.dib.2020.106071

Comparative data analsysis of two multi-drug resistant homoserine lactone and rhamnolipid producing Pseudomonas aeruginosa from diabetic foot infected patient

Data Brief. 2020 Jul 25:32:106071. doi: 10.1016/j.dib.2020.106071. eCollection 2020 Oct.

Authors

Prakhar Srivastava¹, Sankaranarayanan Gomathinayagam¹, Nalini Easwaran¹, Gowri Sankar¹, E Padmavathi¹, Manoharan Shankar¹, Kodiveri M Gothandam¹, Karthikeyan Sivashanmugam¹

Affiliation

¹ School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India.

Abstract

Pseudomonas aeruginosa generally forms strong biofilm during chronic condition of wound. The whole mechanism of biofilm formation works in tandem with quorum sensing circuit of the organism in order to produce virulence. Here we report the draft genome sequence of two diabetic foot ulcer Pseudomonas aeruginosa isolates (VIT PC 7 and VIT PC 9) displaying homoserine lactone, rhamnolipid producing, biofilm phenotype and antibiotic resistance genes related to carbapenem, aminoglycoside, beta- lactamase and tetracycline resistance. The whole genome sequencing library was prepared according to the Oxford Nanopore's SQK-LSK108 kit protocol on Oxford Nanopore's Minion platform. The 7.1 Mb and 6.3-Mb draft genome sequence with GC content of 65.8% and 66.4% respectively provides insight into their resistance mechanism and virulence factors.

Keywords: Biofilm; Diabetic foot ulcer; Efflux pump; Homoserine lactone; Quorum sensing.