Down-regulation of PCK2 inhibits the invasion and metastasis of laryngeal carcinoma cells

Yun Hu; Kun Deng; Meihong Pan; Shanyan Liu; Wenda Li; Jialu Huang; Jinwei Yao; Jianhong Zuo

Down-regulation of PCK2 inhibits the invasion and metastasis of laryngeal carcinoma cells

Am J Transl Res. 2020 Jul 15;12(7):3842-3857. eCollection 2020.

Authors

Yun Hu¹, Kun Deng¹, Meihong Pan², Shanyan Liu², Wenda Li¹, Jialu Huang¹, Jinwei Yao³, Jianhong Zuo^{1

2

3}

Affiliations

¹ Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, The Laboratory of Translational Medicine, Hunan Provincial Key Laboratory of Tumour Microenvironment Responsive Drug Research, Hengyang Medical School, University of South China Hengyang 421002, Hunan, P. R. China.
² The Affiliated Nanhua Hospital of University of South China Hengyang 421002, Hunan, P. R. China.
³ The Third Affiliated Hospital of University of South China Hengyang 421900, Hunan, P. R. China.

PMID: 32774739
PMCID: PMC7407686

Abstract

Laryngeal carcinoma is one of the common malignancies of head and neck. However, the pathogenesis of laryngeal cancer has been not completely clear. To identify the effects of hypoxia on the invasion, metastasis, and metabolism of laryngeal carcinoma, iTRAQ-labeling-with-LC-MS/MS analysis was performed to identify differentially expressed proteins of the SCC10A cells under hypoxia and normoxia, while metabolites were examined by metabolic profiling. 155 proteins and 180 metabolites were identified and the PCK2 protein was selected for validation by Western Blotting. Immunohistochemistry (IHC) was performed to analyze the expression of PCK2 in formalin-fixed paraffin-embedded (FFPE) tissue sections, including laryngeal squamous cell carcinoma tissues from various stages. Collectively, we report that down-regulation of PCK2 inhibits the invasion, migration, and proliferation of laryngeal cancer under hypoxia and down-regulation of PCK2 may be used as a new strategy for laryngeal cancer therapy.

Keywords: Laryngeal cancer; PCK2; hypoxia; invasion; metastasis.